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International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts

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International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts

2020; 13(2): 70-70

Published online April 1, 2020 https://doi.org/10.1016/j.jams.2020.03.007

Copyright © Medical Association of Pharmacopuncture Institute.

Cordycepin inhibits human ovarian cancer by inducing autophagy and apoptosis through Dickkopf-related protein 1/β-catenin signaling

Hwaseung Yoo

East West Cancer Center, Daejeon University Seoul Korean Medicine Hospital, South Korea

http://creativecommons.org/licenses/by-nc-nd/4.0/

Abstract

Background and Aim

Cordycepin, the major active component from Cordyceps militaris, has been reported to significantly inhibit some types of cancer; however, its effects on ovarian cancer are still not well understood. In this study, we treated human ovarian cancer cells with different doses of cordycepin and found that it dose-dependently reduced ovarian cancer cell viability, based on Cell counting kit-8 reagent.

Method and Result

Immunoblotting showed that cordycepin increased Dickkopf-related protein 1 (Dkk1) levels and inhibited β-catenin signaling. Atg7 knockdown in ovarian cancer cells significantly inhibited cordycepin-induced apoptosis, whereas β-catenin overexpression abolished the effects of cordycepin on cell death and proliferation. Furthermore, we found that Dkk1 overexpression by transfection downregulated the expression of c-Myc and cyclin D1. siRNA-mediated Dkk1 silencing downregulated the expression of Atg8, beclin, and LC3 and promoted β-catenin translocation from the cytoplasm into the nucleus.

Conclusion

These results suggest that cordycepin inhibits ovarian cancer cell growth, possibly through coordinated autophagy and Dkk1/β-catenin signaling. Taken together, our findings provide new insights into the treatment of ovarian cancer using cordycepin.

Keywords: apoptosis, Dickkopf-related protein 1, β-catenin, autophagy, cordycepin

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