Real time confocal monitoring of Evans Blue distribution in the mouse head after the dye injection at GB20: (A) the schematic illustration of the injection of Evans Blue into GB20; (B) the fluorescence from Evans Blue in the dorsal part of the mouse head 30 min after the dye injection into GB20; (C) the region of interest (ROI) demonstrating the perivascular pathway of dye spreading from GB20. Images are representative of n = 7 mice.|@|~(^,^)~|@|The histological and confocal analysis of Evans Blue distribution in the mouse head 3h after the dye injection at GB20. (A, F) – the schematic illustration of ROI for the histological (left column) and the confocal (right column) analysis of presence of Evans Blue (red fluorescence, arrowed) in: (B, C) the epaxial musculature; (D, E) the cribriform plate; (G, H) in the ocular part of nasolacrimal duct; (I, J) in the Bowman’s glands. Images are representative of n = 7 mice, scale bars with a magnification of 246.|@|~(^,^)~|@|Perivascular spreading of Evans Blue in the mouse brain after the dye injection at GB20. (A) Representative images of distribution of Evans Blue on the dorsal and ventral surfaces of the brain 3 hours after the dye injection at GB20; (B) The perivascular pathway of Evans Blue spreading in the brain; (C, D) the schematic illustration of Evans Blue distribution along the cerebral veins and arteries, respectively. MCA = Middle cerebral artery; C. Willis = Circle of Willis; PCA = Posterior cerebral artery; BA = Basilar artery; SSS = Superior sagittal sinus; TS = Transverse sinus. Red color, Evans Blue; green color, FITC-dextran; (E) The intensity of the fluorescent signal from Evans Blue in each mouse.

J Acupunct Meridian Stud 2022;15:43~49 https://doi.org/10.51507/j.jams.2022.15.1.43