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  • Research Article2021-10-31

    Comparison of the Effects of Electroacupuncture and Melatonin on Nerve Regeneration in Experimentally Nerve-Damaged Rats

    Yasemin Özkan1,*, Mehmet Turgut2, Yasemin Turan1, Mehmet Dinçer Bilgin3, Sinem Sari4, Mustafa Yilmaz3, Yiğit Uyanikgil5, Mahmut Alp Kiliç3, Derya Tanriöver5, Zehra Seznur Kasar1
    J Acupunct Meridian Stud 2021; 14(5): 176-182 https://doi.org/10.51507/j.jams.2021.14.5.176

    Abstract : Background: Development of methods to accelerate nerve regeneration in peripheral nerve damage is important. Electroacupuncture is a new therapeutic method that combines traditional acupuncture with modern electrotherapy. Melatonin has been shown to reduce nerve damage. Objectives: In this study, we aimed to determine and compare the therapeutic effects of electroacupuncture and melatonin on rat sciatic nerve injury. Methods: A total of 56 adult male Wistar Albino rats were divided into four study groups with 14 animals in each group: intact control (group I), subcutaneous saline (group II), subcutaneous melatonin (group III), and electroacupuncture (group IV). Surgical procedure including unilateral (right) sciatic nerve injury was applied to groups II, III, and IV. Saline and melatonin started immediately after surgery for six weeks, while electroacupuncture was given two weeks after surgery for 3 weeks. Functional and histological assessments were used as outcome measurements. Results: Sciatic nerve damage caused a significant decrease in nerve conduction velocity. Both electroacupuncture treatment and melatonin treatment significantly increased the nerve conduction velocity. Both sciatic functional recovery and histological regeneration were faster in these treatment groups compared to the saline. However, no significant difference was observed between the two treatment groups. Conclusion: Electroacupuncture and melatonin are promising alternative treatment strategies for peripheral nerve damage and can be examined in detail in future studies.

    Abstract
  • Research Article2020-12-01

    Abstract : Background: Despite the adverse consequences of poor sleep during pregnancy, relatively few safe interventions exist for improving sleep among pregnant women. Nonpharmacological interventions are increasingly gaining acceptance. However, the effects of acupressure and acupuncture-like transcutaneous electrical nerve stimulation (ACUTENS) have not been widely reported. Objectives: The purpose of this study is to investigate the effects of low-frequency transcutaneous electrical nerve stimulation, otherwise known as ACUTENS, and acupressure on sleep quality among pregnant women. Methods: Purposive sampling of 42 pregnant women with sleep disorders was conducted, and the participants were randomized into 3 groups, i.e., acupressure, ACUTENS, and usual care. interventions, which were carried out twice weekly for six weeks. The Pittsburgh Sleep Quality index was used to determine the overall sleep quality. Results: The preintervention scores of sleep quality were 12.5 (3.75), 12.5 (4.0), and 13.0 (3.5) in the ACUTENS, acupressure, and usual care groups, respectively. The KruskaleWallis test did not show any significant difference in the preintervention sleep quality scores (H = 0.379, p = 0.827). Participants in the usual care group recorded the least improvement, with a score of 5.0 (2.25), the ACUTENS group recorded a score of 4.5 (3.0), and the greatest improvement was recorded in the acupressure group, with a score of 4.0 (2.2) after 6 weeks of intervention. The KruskaleWallis test did not show any significant difference in the postintervention sleep quality scores among the 3 groups (H = 0.666, p = 0.717). Conclusion: This study showed that ACUTENS and acupressure as an adjunct to usual care are not more effective than usual care alone in improving sleep quality among pregnant women.

    Abstract
  • Abstract : Background: Pain is a major complaint in cancer patients and a global problem that requires medical attention, including pain in cervical cancer. Although pharmacotherapy has been used for the treatment of cancer pain, there are still around 40% cannot be treated only with pharmacotherapy. Objectives: To determine the effects of electroacupuncture (EA) on pain in stage III cervical cancer patients. Methods: Twenty-eight stage III cervical cancer patients were divided into two groups (14 treatments and 14 controls) with randomized control trial design. The treatment group received EA with a frequency of 2/20-25 Hz at points of ST36, SP6, LI4 and LR3 for 30 minutes, while the control group did not receive EA. Both groups were given paracetamol and codeine at the same dose. Assessment was carried out by measuring pain scale (VAS), plasma β-endorphin levels, and quality of life/QoL (EORTC QLQ-C30) before and after therapy. Results: The average reduction in VAS in the treatment group (2.71 ± 1.14) compared to the control group (0.71 ± 1.33; p < 0.001), average increase in plasma β-endorphin levels in the treatment group (88.57 ± 52.46 pg/ml) compared to the control group (12.86 ± 56.76 pg/ml; p = 0.001), and in QoL, there were significant differences in symptom improvement between the treatment and control groups in the domain of fatigue, pain, insomnia and overall QoL (p < 0.05). Conclusion: Medical therapy combined with EA decreased pain scale, increased plasma β-endorphin levels, and improved the QoL for stage III cervical cancer patients.

    Abstract
  • Research Article2021-02-28

    Abstract : Background: Non-alcoholic fatty liver disease (NAFLD) is estimated to be one of the most common diseases affecting the liver because of its high prevalence worldwide. Abnormal lipid profile between NAFLD patients has been reported in several studies. Objectives: This study aimed to evaluate the lipid profile response to electroacupuncture in NAFLD patients. Methods: A total of 60 female patients with NAFLD were included in the study with ages ranged from (30-55) years old. They were divided equally into two groups, group A received electroacupuncture (EA) stimulation at points of; LR14, LR3, ST36, and GB34. And group B received sham acupuncture application in non-acupuncture points. The demographic data and lipid profile such as total cholesterol (TC), serum triglycerides (TG), serum high-density lipoprotein (HDL), serum low-density lipoprotein (LDL) were recorded before and after the study. Results: The study results revealed a significant decrease (p < 0.05) of LDL, TC, TG after 6 weeks of non-interrupted treatment sessions in group A, However HDL showed no significant improvement (p > 0.05). A significant difference was found between posttreatment values of LDL, TC, and TG between both groups. Conclusion: Electroacupuncture can be an effective, simple, and applicable method for the improvement of elevated lipid profiles in NAFLD patients.

    Abstract
  • Conference Abstracts2021-06-30
    JAMS
  • Research Article2020-10-01

    The Effect of Laser Acupuncture on Spasticity in Children with Spastic Cerebral Palsy

    Dian E. Putri 1, *, Adiningsih Srilestari 1, Kemas Abdurrohim 1, Irawan Mangunatmadja 2, Luh K. Wahyuni 3

    Abstract : Background: Spasticity in cerebral palsy is one of the most common disabilities of children in developing countries. Objectives: The objective of this study was to determine the efficacy of laser acupuncture on spasticity in children with spastic cerebral palsy. Methods: This clinical trial was conducted on 60 patients with spastic cerebral palsy at 2 to 10 years. The patients were categorized into two groups: the control group and treatment group. Laser acupuncture was applied on GV20, GV14, LI4, GB34, and LR3 (power 50 mW, 785 nm, 1 Joule, 40 seconds) three times a week for 12 sessions in the treatment group and placebo laser acupuncture on the same points in the control group. The spasticity was measured using the Modified Ashworth Scale before and after complete sessions. Results: The results showed that there was a significant reduction in the Modified Ashworth Scale score in the treatment group compared with the control group (p = 0.003). Conclusions: This study suggest that laser acupuncture on GV20, GV14, LI4, GB34, and LR3 can reduce spasticity for children with spastic cerebral palsy.

    Abstract
  • Clinical Study Protocol2021-12-31

    Abstract : Background: Spasticity is a common motor disorder in adult stroke patients. Injury to the corticospinal tract (CST) is associated with spasticity. Dry needling (DN) has positive impacts on spasticity reduction and improvement in the range of motion (ROM) in stroke patients. This study aims to investigate the effectiveness of DN on the connectivity of the CST and the level of spasticity in adult stroke patients. Methods: This double-blind randomized controlled trial will enroll and randomly assign stroke patients to either the experimental group, for receiving three sessions of DN for the spastic wrist flexors, or the control group, for sham needling. The primary outcome measures will be diffusion tensor imaging and the Modified Modified Ashworth Scale score to assess CST connectivity and wrist flexor spasticity, respectively. The Box and Block Test and standard goniometry are the secondary outcome measures to assess hand dexterity and active and passive wrist ROM, respectively. Measurements will be taken both before and after the intervention. Discussion: The results of this study will provide important evidence of the effects of DN on CST connectivity, spasticity, and arm function in adult stroke patients. Trial registration: This trial is registered in the Iranian Registry of Clinical Trials (IRCT) (https://www.irct.ir; IRCT20191208045649N1).

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  • Research Article2020-06-01

    Effectiveness of Manual and Electrical Acupuncture for Chronic Nonspecific Low Back Pain: A Randomized Controlled Trial

    Josielli Comachio 1, *, Carla C. Oliveira 1, Ilton F.R. Silva 1, Mauricio O. Magalhães 1, 2, Amélia P. Marques 1

    Abstract : Background: Low back pain is a common condition that can be effectively treated by acupuncture. However, several treatment point prescriptions and further electrical needle stimulation (i.e., local acupoints, distal acupoints, and sensitized acupoints) may be used. There is an implicit yet unexplored assumption about the evidence on manual and electrical stimulation techniques. Objective: The present study aims to identify effectiveness of electroacupuncture (EA) and manual acupuncture (MA) on pain and disability in patients with chronic nonspecific low back. Methods: This study is a randomized controlled clinical trial. Sixty-six patients between 20 and 60 years of age with non-specific chronic low back pain experiencing low back pain lasting for at least the previous three months and ≥3 points on a 10 numerical analogic scale. Patients diagnosed with chronic LBP were assigned to receive either 12 sessions of MA or EA. The primary outcomes measurements were pain intensity on Numeric Rating Scale and disability by Roland Morris Disability Questionnaire. Results: The participants reported improvements post-treatment to pain intensity and disability respectively; however, no differences between groups were observed. Regarding the secondary outcomes, we observed a between-group difference only for kinesiophobia in favor of the manual acupuncture group (difference = -4.1 points, 95% CI = -7.0 to -1.1). The results were maintained after 3 months of follow-up. Conclusion: The study provides evidence that EA is not superior to MA treatment. Both therapies had similar efficacy in reducing pain and disability for chronic nonspecific low back pain.

    Abstract
  • Research Article2020-06-01

    Abstract : Background and objectives: Needle insertion pain during spinal anesthesia is an unpleasant experience for patients. This study aimed to investigate the effects of Transcutaneous Electrical Nerve Stimulation (TENS) on the pain intensity during the insertion of spinal needles in patients undergoing spinal anesthesia. Materials and methods: In a double-blind clinical trial, 60 candidates for elective Trans Ureteral Lithotripsy surgery under spinal anesthesia were randomly divided into intervention and control groups. The electrodes of the TENS device were placed in the space between L3-L4 and L5-S1 vertebrae. The intensity of pain during insertion of the spinal needle by Visual Analog Scale and the frequency of attempts were recorded. Results: The mean age of the study samples was 34.26 ± 5.07 and 32.8 ± 5.28 in the control and intervention group, respectively. The pain intensity during insertion of spinal needles was less significant in the intervention group compared to the control group (p = 0.001). The number of attempts to insert the spinal needle between the two groups was not statistically significant (p = 0.51). The duration of spinal anesthesia implementation procedure by physician in the intervention group was significantly shorter than that of the control group (p = 0.001). Conclusion: The use of TENS effectively reduced the pain of spinal needle insertion. Considering these beneficial effects, it is suggested that this procedure be used to relive pain in patients with spinal anesthesia.

    Abstract
  • Abstract : Primary dysmenorrhea is defined as cramping pain in the lower abdomen with no pelvic diseases, and it has a high prevalence in many countries. Acupressure is a widely used complementary treatment method for primary dysmenorrhea. This review examined experimental studies to determine the effects of acupressure on primary dysmenorrhea using the databases PubMed, Google Scholar, and CINAHL with the keywords “Acupressure” and “Dysmenorrhea”. There were 2227 records in the databases, and 330 articles were published between 1989 and March 2020. Experimental studies in the English language were reviewed according to the PRISMA guidelines. This review included 28 published studies that were assessed using the Jadad score for quality. The studies were categorized as studies of acupressure at the LR3 point (n = 4), at the SP6 point (n = 9), at auricular points (n = 5), at multiple points (n = 8), and with devices (n = 2). Moreover, studies of self-acupressure (n = 9) were identified. The studies demonstrated that acupressure could reduce menstrual symptoms, the severity and duration of menstrual pain, distress, and anxiety. Furthermore, it helped improve the quality of life and well-being of patients and provide psychological support and self-care. Acupressure is an inexpensive, easy-to-apply, and non-pharmacological treatment and is useful for reducing primary dysmenorrhea, and women can apply this method anywhere by themselves. However, high-quality randomized controlled trials with larger samples are necessary to establish the evidence for acupressure as an effective intervention.

    Abstract

Journal Info

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
April, 2022
Vol.15 No.2

pISSN 2005-2901
eISSN 2093-8152

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  • Research Article2020-12-01

    Abstract : Background: Anxiety is a common complaint of patients before diagnostic or therapeutic invasive procedures, especially before open-heart surgery. The most well-known method to reduce anxiety is the use of sedatives, which have pronounced side effects. Objectives: The purpose of this study was to determine the effect of acupressure on anxiety in patients undergoing open-heart surgery. Method: This is a randomized clinical trial study conducted on 90 patients who were candidates for open-heart surgery. The patients were randomly assigned into either intervention or control groups. Acupressure intervention was applied at three real acupoints over two consecutive days in the intervention group. The control group received acupressure on sham points. We used Spielberger State-Trait Anxiety Inventory to assess anxiety in our study. Results: The results showed that before acupressure, there was no statistically significant difference between state anxiety scores and intergroup traits, and this difference was only significant in state anxiety after the second intervention. State and trait anxiety were significant before and after the intervention in the test group, respectively include (p < 0.001) (p = 0.01), but these changes in the control group did not show a statistically significant difference. After completing the second phase of the intervention at the actual sites, systolic blood pressure (p = 0.007) and heart rate (p = 0.001) decreased significantly. However, acupressure did not have a significant effect on diastolic blood pressure in any of the groups. Conclusion: Based on the results of this study, the application of acupressure in patients who are candidates for open-heart surgery can reduce their state anxiety. Further larger-scale and rigorous studies are warranted.

    Abstract
  • Research Article2021-04-30

    Abstract : Background: Diabetes mellitus (DM) is a chronic metabolic disease characterized by increased blood sugar levels. The current management of DM to date has a target of controlling blood glucose, but the therapy cannot be separated from long-term drug side effects. Acupuncture can be an option as an adjunct therapy for DM. Objectives: The purpose of this study was to compare the effectiveness between manual acupuncture and laser acupuncture. Methods: This study was a randomized control experimental study with a pretest and posttest design using 24 male Sprague-Dawley rats divided into 4 groups: a normal group, a diabetes group, an acupuncture group, and a laser group. Manual acupuncture and laser acupuncture were performed 6 times in two weeks. Fasting blood glucose (FBG) levels, the cell density of Langerhans islets, and side effects were assessed and compared among the 4 groups. Results: The highest mean cell density of Langerhans islets was found in the laser and acupuncture group, and the lowest was found in the diabetes group. In the post hoc analysis, the normal, acupuncture, and the laser groups had a significantly higher mean cell density than the diabetes group. The lowest mean FBG level was in the laser group, followed by the acupuncture group, and the highest was in the diabetes group, but this difference was not significant. There were no serious side effects from the use of manual acupuncture or laser acupuncture. Conclusion: Both manual acupuncture and laser acupuncture can improve the histological findings of Langerhans islets in type 2 diabetic rats, and both are safe to use.

    Abstract
  • Research Article2021-08-31

    Heart Rate Variability and Psychometric Analysis in Patients with Hyperactive Heart Fire Syndrome

    Oliverio Medina Martínez1, Ruben Fossion3,4, Yolanda García Piceno1,2, Rosa E. Lopez-Gomez1,2, Emma López-Espinosa1,2, Ismael Jiménez-Estrada5, Salvador Quiroz-González1,2,*
    J Acupunct Meridian Stud 2021; 14(4): 137-148 https://doi.org/10.51507/j.jams.2021.14.4.137

    Abstract : Background: Hyperactive heart fire syndrome is characterized by anxiety, insomnia, dream-disturbed sleep, tongue ulcers, heat in the hands, and palpitations. However, syndrome differentiation is often subjective due to a lack of objective, quantifiable variables. Objectives: To identify changes in heart rate variability (HRV) and psychometric analysis in patients with hyperactive heart fire syndrome. Methods: Healthy controls (n = 33) were compared to patients with hyperactive heart fire syndrome (n = 48) from the Integrative University Clinic of the State University of Ecatepec Valley (CIU-UNEVE). Physiological outcome measures included heart rate (HR), the standard deviation of the normal-to-normal heartbeat intervals (SDNN), low (LF) and high frequency (HF) power, and the LF/HF ratio. Psychometric outcome measures included the Athens Insomnia Scale (AIS) and the Hamilton Anxiety Rating Scale (HARS). Results: Compared to controls, hyperactive heart fire patients had higher HR (9.6 ± 2.62%), LF (22 ± 4.21%) and LF/HF ratio (23 ± 3.14%), and lower SDNN (21 ± 2.33%) and HF (18 ± 4.61%). Patients showed increased anxiety, both with somatic (33 ± 11.2%) and psychic symptoms (39 ± 10.5%) with more difficulty falling asleep (47 ± 9.9%) and diurnal impact of sleep (31 ± 9.6%). Conclusion: Hyperactive heart fire patients may have a sympathovagal imbalance due to a reduced parasympathetic tone and/or adominant sympathetic tone, which may be at the origin of the observed symptoms of insomnia and anxiety.

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  • Research Article2021-04-30

    Abstract : Background: Migraine is a very common neurobiological headache disorder caused by an increased irritability of the central nervous system. Acupuncture as a complementary medicine has been suggested as one of the treatments for migraine headaches; however, the findings are conflicting. Objectives: Therefore, the aim of this study was to evaluate the effect of acupuncture with auricular semi-permanent (ASP) needles on migraine headaches. Methods: In this single-blind randomized controlled trial, 80 patients with migraine were selected and divided into two groups. The intervention group was treated with auricular ASP needles in the active points of the ear, and the control group only received routine treatments. Pain score, frequency of migraine headaches, duration of headaches, severity of nausea and vomiting, and patient satisfaction were compared between the two groups for four weeks after the intervention. Results: Our results showed that the level of pain (4.72 ± 2.53, 2.13 ± 1.76 times) and the frequency of migraine headaches (8.98 ± 8.58 hours) from the second week after the intervention in the ASP group were much lower than those in the control group (p < 0.05). However, pain incidence and ear inflammation in the ASP group were negligible and did not differ significantly from those in the control group (p > 0.05). Conclusion: Auricular acupuncture could be considered as a promising complementary therapy along with other standard migraine therapies for the prevention and treatment of migraine headaches.

    Abstract
  • Research Article2021-06-30

    Abstract : Background: Nursing students experience clinical stress frequently and severely. The application of acupressure is reported to be effective in stress management.Objectives: This study was conducted to determine the effects of acupressure on reducing the stress of nursing students in clinical practice. Methods: This study was carried out using a single-blind randomized controlled experimental design. The experimental and control groups were randomly determined by using a previously prepared randomization checklist. A Participant Information Form, VAS, and the State Anxiety Inventory were applied to all students before practice. Acupressure was performed on the HT7 point and Yintang point (EX-HN3), respectively, every five minutes for a total of 30 minutes in the experimental group. Results: The level of stress experienced by the students in the experimental group before the intervention according to VAS was 6.95 ± 1.57, and it was determined as 2.82 ± 1.94 after the third application (p < 0.05). The mean clinical stress score before the application was 46.54 ± 3.81, and after the 3rd week of application, it was 25.15 ± 5.26 (p < 0.05). It was observed that the students' stress levels decreased in all measurements made after the acupressure intervention. Conclusion: This study determined that acupressure effectively reduces the stress levels of nursing students, and it may be applied in clinical stress management.

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  • Research Article2021-02-28

    Effect of Remote and Local Acupuncture Points on Periarthritis of Shoulder: A Comparative Study

    Kumaresan Poorna Chandran1,*, Prabu Poorna Chandran2, Naveena Arumugam3, Sendhilkumar Muthappan4

    Abstract : Background: Periarthritis of shoulder is a painful condition of the shoulder, affecting 2-3% of the general population and 20% of diabetic patients. Acupuncture is a widely practiced traditional Chinese medicine. Recent evidence shows that it alleviates shoulder pain with different needling techniques. Objectives: The present study is to compare the efficacy of remote and local points on PAS. Methods: 60 subjects were randomly assigned into two groups, remote acupuncture group (n = 30) and local acupuncture group (n = 30). Both groups were assessed at baseline and at the end of 12 sessions. Shoulder pain and its disability index (SPADI) and (ROM) were measured using goniometer. The intervention was given weekly thrice on alternate days for four weeks with 20 min for each session. Results: The result shows that both remote and local acupuncture points were beneficial in the pain management and rage of motion when compared within the group. The effects of acupuncture at remote acupoints were better than those at local acupoints in SPADI and ROM when compared between two groups. Conclusion: In treatment of periarthritis of shoulder remote acupuncture points may have higher therapeutic value when compared to local points.

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