Images of lymph node in the vena cava of a rabbit. (A) Before LPS injection to lymph node. (B) After LPS injection to lymph node. Images of staining lymph node. (C) After AB injection. (D) Floating PV inside a LV. (E) Image of an isolating PV from LV. (F) Image of an isolated PV. White dot circle lines indicate lymph node and white and sky color arrows indicate LV and PV, respectively. AB = alcian blue; LPS = lipopolysaccharide; LV = lymphatic vessel; PV = primo vessel.|@|~(^,^)~|@|Expression of LEC markers in LV including PV, PV under inflammation was revealed and both LV and PV in acupoint stimulation after LPS was also changed their gene expression. Gene expressions for LV and PV by LPS (+) treatment and before (−) and after (+) AES at two acupoints ST36 and LI04. Samples were harvested and subjected to qRT-PCR. Each value is the average of duplicated experiments (at least 2 per experiment). * and ^# indicate significant difference versus Group 1 (LV including PV under LPS) and Group 2 (only PV under LPS) (* and ^#p < 0.05, *** and ^###p < 0.001). LEC = lymphatic endothelial cell; LPS = lipopolysaccharide; LV = lymphatic vessel; PV = primo vessel; qRT-PCR = quantitative reverse transcriptase–polymerase chain reaction.|@|~(^,^)~|@|Expression of LEC markers in LV including PV, PV under inflammation was displayed, and both LV and PV in AES after LPS was also changed their gene expression. Gene expressions for LV and PV by LPS (+) treatment and before (−) and after (+) AES at two acupoints ST36 and LI04. Samples were harvested and subjected to qRT-PCR. Each value is the average of duplicated experiments (at least 2 per experiment). * and ^# indicate significant difference versus Group 1 (LV including PV under LPS) and Group 2 (only PV under LPS) (* and ^#p < 0.05, ** and ^##p < 0.01, *** and ^###p < 0.001). AES = acupuncture electric stimulation; LEC = lymphatic endothelial cell; LPS = lipopolysaccharide; LV = lymphatic vessel; PV = primo vessel; qRT-PCR = quantitative reverse transcriptase–polymerase chain reaction.

2019;12:3~10 https://doi.org/10.1016/j.jams.2018.03.003