Visualization of the PVS in the internal lymph vessel between the inguinal lymph node and axillary lymph node. (A) A schematic diagram of the vein vascular duct and lymphatic vessel system in a rabbit. The inguinal lymph node, the abdominal lymph node, and the axillary lymph node were located near the subclavian, the cava vena, and the iliac veins, respectively. (B) The right and left axillary node, the abdominal node, and the right and left inguinal node into which AB solution was injected which became blue as the AB flowed through the lymph duct. (C) Three magnification views of the blue-stained PVS in the lymph vessel. Note that the primo vessel was much thinner than the lymph vessel. The thickness of the lymph vessel was 100–150 μm, whereas that of the primo vessel was 20–30 μm.|@|~(^,^)~|@|Scatter plots of gene expression for combinations of three different experimental groups: Group 1(primo vessel), Group 2(primo vessel + lymph vessel), and Group 3(lymph vessel) by RNA-Seq data analysis. (A) Group 1 vs Group 3, (B) Group 1 vs Group 2 and (C) Group 3 vs Group 2. The significant genes form the distribution of scattered genes noticed by the red and blue color dots.|@|~(^,^)~|@|Expression level (FPKM) of 10 genes for Group 1 (primo vessel) vs Group 3 (lymph vessel). Samples were harvested and subjected to RNA-Seq. Each value is the average of duplicated experiments (at least 2 per experiment). The p-value for each gene is < 0.05. FPKM, Fragments Per Kilobase of exon per Million.|@|~(^,^)~|@|Expression level (FPKM) of 10 genes for Group 1 (primo vessel) vs Group 2 (primo + lymph vessel). Samples were harvested and subjected to RNA-Seq. Each value is the average of duplicated experiments (at least 2 per experiment). The p-value for each gene is < 0.05. FPKM, Fragments Per Kilobase of exon per Million.|@|~(^,^)~|@|Expression level (FPKM) of 10 genes for Group 3 (lymph vessel) vs Group 2 (primo + lymph vessel). Samples were harvested and subjected to RNA-Seq. Each value is the average of duplicated experiments (at least 2 per experiment). The p-value for each gene is < 0.05. FPKM, Fragments Per Kilobase of exon per Million.

2019;12:11~19 https://doi.org/10.1016/j.jams.2018.10.008