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  • Abstract : This case report chronicles the treatment of a case of plantar fasciitis with acupuncture and tui na for more than six clinic visits. The patient was a 41-year-old female with plantar fasciitis for two months. A variety of acupuncture and electroacupuncture protocols were used at first, with no benefit. After the fourth and fifth visits, however, the patient experienced total relief of pain and a significant reduction in stiffness that persisted through her final visit two weeks later. These later treatments included the yuan-luo (origin-connecting) acupuncture point pair KI 3 and BL 58, which is one possible explanation for their effectiveness. The yuan-luo pair is a classical point combination with much theory behind it, yet no studies have been published regarding its practical uses in clinic. This case is an example of one practical application of the pair; further examples are needed.

    Abstract
  • Abstract : Role of electroacupuncture (EA) in refractory unilateral sensorineural hearing loss (SNHL) remains unclear but might be promising for the Meniere's disease. Two cases of unilateral SNHL who were unresponsive to conventional treatment of sudden SNHL showed complete recovery after receiving EA therapy. The first case was a 46-year-old woman who received EA in the seventh month after the acute onset of sudden right hearing loss and tinnitus. She had mild-to-moderate degree of SNHL at high frequencies in the right ear with episodic vertigo. The second case was a 55-year-old woman who received EA in the sixth year after developing sudden SNHL in the right ear. Before the EA began, her pure tone average of the affected ear was 45 dB and the phonetically balanced score was 88%. The regimen for both patients included 12 sessions of EA over four weeks at the main acupoints (Tinggong (SI 19), Ermen (TE 21), Qimai (TE 18) and Yifeng (TE 17) on the affected ear and the adjuvant acupoints (Zhongzhu (TE 3), Hegu (LI 4), Qihai (CV 6), Guanyuan (CV 4), Taixi (KI 3), and Taichong (LIV 3)). Both patients regained their normal hearing thresholds three weeks after the first EA. No adverse events were observed. Hence, EA may be a useful additional therapy in unilateral SNHL, even at the late phase when other treatments have failed because the possibility of Meniere's disease cannot be excluded.

    Abstract
  • Abstract : The primo vascular system (PVS) has been difficult to detect due to its small diameter and translucent features of the thread-like network. Thus, the developed methods to find and take out PVS were to use contrast-enhancing dyes including Alcian blue, Trypan blue, and Janus green B. To use as a detector to PVS and a biological tool for functional study of PVS, monoclonal antibodies (mAbs) against PVS of rat were generated by various techniques, such as cell fusion, enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), screening of hybridoma, immunofluorescence microscopy (IF). Among 16 mAbs generated, 4 representative mAbs were characterized with their specificities in ELISA, WB, and IF. α-rPVS-m1-1 and α-rPVS-m4-6 had strong binding affinities to PVS in both ELISA and WB but did not show specificities at all in IF. On the contrary, α-rPVS-m3-2 and α-rPVS-m3-4 almost did not respond in WB but had strong binding affinities in ELISA and specificities in IF. Two mAbs stained predominantly at extracellular matrix and cell membrane of PVS of rat in IF. Thus, α-rPVS-m3-2 and α-rPVS-m3-4 can be used as a tool in discriminating PVS from blood vessel (BV) and lymphatic vessel (LV) and other similar tissues of rat in IF.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Emerging data indicates that not all chronic pain patients with a given diagnosis have the same underlying pathophysiology. This is particularly relevant for the widespread pain condition known as fibromyalgia (FM) wherein existing pharmacologic and non-pharmacologic treatments are suboptimal: only 30-40% of patients have a clinically meaningful reduction in their pain. Varying degrees of pain pathophysiology are also likely relevant for acupuncture as thousands of patients are often needed to determine statistical significance between sham and verum groups in randomized controlled clinical pain trials. I will present data suggesting that quantitative sensory testing and needling sensations obtained at baseline, can be used to identify different biotypes of FM patients that respond differentially to sham and verum acupuncture. More sensitive FM patients respond better to sham acupuncture whereas less sensitive patients respond better to verum. This type of data could improve clinical trial power by selectively identifying the right type of patient at baseline that is likely to respond to the right type of acupuncture treatment. Thus supporting a more personalized approach to the treatment of chronic pain with acupuncture.

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  • Abstract : The institute of Cannabis Research (ICR) was established in 2016 through a unique partnership between Colorado State University-Pueblo, the State of Colorado, and Pueblo County. The mission of the ICR is to generate new knowledge and understanding of cannabis through innovative research, education, and dissemination of results and information. This presentation will include background information on the ICR and its activities, a discussion of trends in cannabis research in the United States, and results from some research projects supported through the ICR. The first project focuses the development of an efficient extraction method for the recovery of the cannabinoid cannabidiol (CBD) from industrial hemp (< 0.3% Δ9-tetrahydrocannabinol) using pressurized liquid extraction (PLE). This unique PLE method includes a dual extraction process in which the hemp undergoes a pre-extraction with water to promote decarboxylation of cannabidiolic acid (CBD-A) to CBD, without significant loss of neutral cannabinoids, followed by PLE using ethanol to extract the CBD. This PLE method can be employed to efficiently carry out decarboxylation of other acidic cannabinoids to their neutral counterparts. A second project that will be discussed is an interrupted time series analysis was conducted to assess the impact of Colorado’s legalization of both medical (2000) and recreational (2014) cannabis over the time period 1999 - 2018. Both were associated with a reduction of opioid overdose mortality rates. This reduction represents a reversal of the upward trend in opioid-related deaths in Colorado. The final projects that will be discussed aim at showing mechanisms which underlie effects of various cannabinoids on learning and memory in a rodent model. Our published results have shown prominent effects of Cannabidiol on the fundamental mechanisms of learning and memory, and work in progress is showing similar effects of HU-211, a synthetic glutamate receptor inhibitor. This basic medical research involves behavioral, pharmacological, electrophysiological and histological methods and has relevance for understanding appropriate medical applications for cannabinoids to treat brain disorders ranging from Alzheimer’s disease to Post Traumatic Stress Disorder.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Objectives This study is related to stimulate of mesenchymal stem cell (MSC) release into blood by use of electroacupuncture (EA) at specific acupoints. Methods EA was performed in rats using LI4, LI11, GV14, and Baihui (rats). Results Through some methods such as functional magnetic resonance imaging (fMRI) and pharmacological disinhibition of hypothalamus, the activation of the sympathetic nervous system resulted in a release of MSC into the circulation. In addition, EA treatment in rats that had partial rupture of the Achilles tendon induced analgesia, increasing interleukin-10 levels and remodeling. Conclusion EA as low risk, and very effective method should be used to support the repair of tissue wound by increasing of the circulating MSC.

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  • Research Paper2019-08-01

    Changes in Heart Rate Variability in Patients with Spleen-Qi Deficiency Syndrome

    Agnese Olivera-Toro1, Rubén Fossion34, Lei Li56, Rosa E. López-Gómez1, Emma López-Espinosa1, Ismael Jiménez-Estrada7, Salvador Quiroz-González12*

    Abstract : Many functional diseases are related to dysautonomia, and heart rate variability has been used to assess dysautonomia. However, heart rate variability has not been studied in Spleen-Qi deficiency syndrome (SQDS). Healthy volunteers (n = 37) and patients with SQDS (n = 67), recruited from the Clinic of the State University of Ecatepec Valley were included in the study. Outcome measures were average heart rate, standard deviation of the normal-to-normal heartbeat intervals, low frequency (LF), high frequency (HF) power, and the LF/HF ratio. Also, intestinal peristalsis, gastrointestinal symptoms (GSs), fatigue, and level of attention were measured. Standard deviation of the normal-to-normal heartbeat intervals (17 ± 2.3%) and HF (14 ± 3.1%) were lower in SQDS patients (17 ± 1.3%) than in healthy volunteers. SQDS patients had higher heart rate, LF power, LF/HF ratio, and fatigue scores (9.6 ± 1.12%, 16 ± 2.1%, 22 ± 3.8%, and 21 ± 4.1%). The fatigue correlated positively with the LF/HF ratio and negatively with HF power. The SQDS group had lower concentration performance (16.2 ± 1.9%) in the d2 test. The intestinal peristalsis showed a reduction (15 ± 1.3%) as compared with control. GS score and peristalsis correlated negatively with HF. Our results suggest that the pathology of SDQS could be associated with a low vagal tone which causes a decrease in peristalsis, increased fatigue, reduced attention, and appearance of GSs.

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  • Research Paper2019-10-01

    Pressuring of Acupoints as a Complement to the Diagnosis of Stifle Diseases in Horses

    Luiza P.R. Mariani1, Flávia Sampaio2, Andressa B. Silveira23, Luis F. Bastos4, Saulo H. Weber1, Pedro V. Michelotto1*

    Abstract : Diseases of the stifle joint remain a challenge for veterinarians. The objective of this study was to achieve a valuable acupuncture suggestive diagnosis to be considered for stifle joint diseases in horses. Thirty-nine nonlame horses involved in different activities were assessed. Acupuncture was independently performed by two evaluators. Reactions of the animal when pressurizing the point suggestive of stifle disease (PSSD), Bladder-20 and/or Bladder-21, were considered as the inclusion criteria for inclusion in the stifle group (SG, n = 31), and the animals with no reactions were assigned to the control group (n = 8). Radiographic and ultrasonographic examinations were performed and evaluated by two independent professionals blinded to the group allocation. Thermographic examination of the PSSD and stifles was also performed, after acclimatization. The ultrasound scores and radiographic findings were higher in the SG than in the control group. Thermography evidenced increased temperature in the PSSD and stifles in the SG. The minimum acupuncture diagnostic criteria for stifle joint disease had a sensitivity of 87.5% and a specificity of 57.0%, and the addition of the acupoints Gallbladder–dorsal tuber coxae, Gallbladder-27, and Spleen-13 to the minimum diagnostic criteria improved sensitivity and specificity. In conclusion, assessing the reaction at the demonstrated acupoints can facilitate a diagnosis of a potential stifle lesion.

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  • Research Article2019-06-01

    Abstract : The aim of this study was to investigate the effects of scalp acupuncture and electrostimulation, combined or not, in a disuse model consisted of early sensorimotor restriction in rats. Male Wistar pups received sensorimotor restriction from the second postnatal day (P2) until P28. Animals were divided into five different groups (n = 6): control (CT), sensorimotor restricted (SR), acupuncture (AC), electrostimulation (EL), and electroacupuncture (AC+EL). Experimental animals received sham, acupuncture, or electrical stimulation, combined or not, of two scalp regions for 7 days (P29-P35). Before treatment period (P29) and after treatment (P36), animals were evaluated with the narrow suspended bar, horizontal ladder, and stride length tests. SR animals had worse performance in the narrow suspended and horizontal ladder tasks compared with SR animals at P29 (p ≤ 0.005). Significant improvements were observed in both tasks in AC, EL, and EL+AC groups comparing P29 and P36 (p 

    Abstract

Journal Info

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
February, 2021
Vol.14 No.1

pISSN 2005-2901
eISSN 2093-8152

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  • Research Paper2019-12-01

    Wet Cupping—Traditional Hijamah Technique versus Asian Cupping Technique in Chronic Low Back Pain Patients: A Pilot Randomized Clinical Trial

    Sulaiman M. Al-Eidi1, Ashry Gad Mohamed2, Raid A. Abutalib3, Abdullah M. AlBedah1, Mohamed K.M. Khalil4*

    Abstract : To evaluate the feasibility of comparing the effect of the traditional Hijamah and the Asian wet cupping techniques in the management of patients with chronic low back pain (CLBP), a randomized clinical trial comparing traditional and Asian wet cupping techniques for CLBP was conducted in two secondary care hospitals in Saudi Arabia. Seventy eligible participants with CLBP were randomized to receive one session of wet cupping using either Asian technique (34 patients) or traditional Hijamah technique (36 patients). Cupping was performed at four sites of the bilateral bladder meridian (BL23, BL24, and BL25). The numeric rating scale, Present Pain Intensity, and Oswestry Disability Questionnaire scores were measured immediately after intervention, at seven days, and 14 days after intervention. In both groups, there was a significant decrease in the numeric rating scale, Present Pain Intensity, and Oswestry Disability Questionnaire scores, immediately after intervention, at seven days, and 14 days after intervention. However, there was no significant difference between the two groups across all the outcome measures up to 14 days after intervention. The study did not show a superiority of one technique compared with the other. Longer follow-up periods and more than one cupping session may be needed to evaluate the difference, if any, between both the techniques. Trial Registration NCT02012205.

    Abstract
  • Research Article2020-08-01

    Abstract : Background: The primo vascular system (PVS) is a novel network composed of primo nodes (PNs) and primo vessels (PVs). Currently, its anatomy is not fully understood. Objectives: The aim of this study was to elucidate the three-dimensional PNePV structure. Methods: Organ-surface PVS tissue was isolated from healthy and anemic rats. The tissues were analyzed by X-ray microcomputed tomography (CT), hematoxylin and eosin staining, and scanning electron microscopy. Results: From CT images, we identified one or more bundles in a PV. In the PN, the bundles were enlarged and existed in isolation and/or in anastomosis. The transverse CT images revealed four areas of distinct intensities: zero, low, intermediate, and high. The first two were considered to be the sinuses and the subvessels of the PVS and were identified in the hematoxylin and eosinestained PN sections. The enlargement of the PN from anemic rats was associated with an increase in the intermediate-intensity area. The high-intensity area demarcated the bundle and was overlapped with the mesothelial cells. In scanning electron microscopy, the PV bundles branched out, tapering down to a single bundle at some distance from the PN. Each bundle was composed of several subvessels (~5 µm). Clustered round microcells (1-25 µm), scattered flat oval cells (~15 µm), and amorphous extracellular matrix were observed on the surface of the PVS tissue. Conclusions: The results newly showed that the primo bundle is a structural unit of both PVs and PNs. A bundle was demarcated by high CT intensity and mesothelial cells and consisted of multiple subvessels. The PN bundles contained also sinuses.

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  • Protocol2020-10-01
    JAMS

    Acupuncture and Dry Needling in the Reduction of Peripheral Acute Fatigue Induced in the Biceps Braquii: Protocol for a Single-blinded Randomized Controlled Clinical Trial

    Gabriel Barreto Antonino 1, *, Ana Paula de Lima Ferreira 2, Eduardo José Nepomuceno Montenegro 3, Alberto Galvão de Moura Filho 2, Arthur Felipe Freire da Silva 3, Maria das Graças Rodrigues de Araújo 2

    Abstract : The present study aims to propose a protocol to verify the efficacy and acute effects of traditional Chinese acupuncture, dry needling, and the rest in peripheral acute fatigue (PAF) induced by intermittent isometric contractions of the nondominant biceps brachii (BB) of nonphysically active men in a randomized, single-blind clinical trial assessed with surface electromyography, contraction time in seconds, infrared thermal imaging, and visual analog scale applied to the PAF. These instruments will evaluate the median frequency, endurance time, temperature (°C), and perceived fatigue in BB of the volunteers. The measurements will be collected in four moments (Test 0, 01, 02, and 03) divided between the beginning and the end of two sets of exercises (Exercises 01 and 02) of intermittent isometric contractions.

    Abstract
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  • Protocol2020-10-01
    JAMS

    Acupuncture and Dry Needling in the Reduction of Peripheral Acute Fatigue Induced in the Biceps Braquii: Protocol for a Single-blinded Randomized Controlled Clinical Trial

    Gabriel Barreto Antonino 1, *, Ana Paula de Lima Ferreira 2, Eduardo José Nepomuceno Montenegro 3, Alberto Galvão de Moura Filho 2, Arthur Felipe Freire da Silva 3, Maria das Graças Rodrigues de Araújo 2

    Abstract : The present study aims to propose a protocol to verify the efficacy and acute effects of traditional Chinese acupuncture, dry needling, and the rest in peripheral acute fatigue (PAF) induced by intermittent isometric contractions of the nondominant biceps brachii (BB) of nonphysically active men in a randomized, single-blind clinical trial assessed with surface electromyography, contraction time in seconds, infrared thermal imaging, and visual analog scale applied to the PAF. These instruments will evaluate the median frequency, endurance time, temperature (°C), and perceived fatigue in BB of the volunteers. The measurements will be collected in four moments (Test 0, 01, 02, and 03) divided between the beginning and the end of two sets of exercises (Exercises 01 and 02) of intermittent isometric contractions.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01

    Abstract : Respiratory inflammation is a frequent and fatal pathologic state encountered in veterinary medicine. Although diluted bee venom (dBV) has potent anti-inflammatory effects, the clinical use of dBV is limited to several chronic inflammatory diseases. The present study was designed to propose an acupoint dBV treatment as a novel therapeutic strategy for respiratory inflammatory disease. Experimental pleurisy was induced by injection of carrageenan into the left pleural space in mouse. The dBV was injected into a specific lung meridian acupoint (LU5) or into an arbitrary non-acupoint located near the midline of the back in mouse. The inflammatory responses were evaluated by analyzing inflammatory indicators in pleural exudate. The dBV injection into the LU5 acupoint significantly suppressed the carrageenan-induced increase of pleural exudate volume, leukocyte accumulation, and myeloperoxidase activity. Moreover, dBV acupoint treatment effectively inhibited the production of IL-1β, but not TNF-α in the pleural exudate. On the other hand, dBV treatment at non-acupoint did not inhibit the inflammatory responses in carrageenan-induced pleurisy. The present results demonstrate that dBV stimulation in the LU5 lung meridian acupoint can produce significant anti-inflammatory effects on carrageenan-induced pleurisy suggesting that dBV acupuncture may be a promising alternative medicine therapy for respiratory inflammatory diseases.

    Abstract
  • Impact Shot2020-10-01

    Herbal Transformation by Fermentation

    Chang-Gue Son 1, *, Sam-Keun Lee 2, In-Kyu Choi 2, Eun-Su Jang 1, Kee-Jung Bang 3

    Abstract : We herein show a dramatic change of herbal properties of the composition as well as function via fermentation of Cynanchi atrati Radix (family Asclepiadaceae). Cynanchi atrati Radix showed a high cytotoxicity against B16-F10 melanoma cell line, but the function of Cynanchi atrati Radix was completely changed into anti-melanin activity at very low concentration after Lactobacillus -fermentation. In addition, the compounds were drastically changed as shown in HPLC-based profile. Furthermore, this transformation has been achieved by only Lactobacillus -fermentation. This study proposes an strategy which we need to consider in the herb-derived material researches including pharmacopuncture.

    Abstract
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