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  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Although researches on the primo vascular system (PVS) were mainly done with mice and rats there have been some experiments with other animals such as rabbits, dogs, pigs, sheep, cow and horses. Especially rabbits were used by BongHan Kim, the discoverer of the PVS, and in the beginning of the author’s laboratory because of the size of the animal is convenient for the observation of the PVS. Other animals than rabbits, rats and mice were only sporadically used for the PVS of some specific organs. The sizes and structures of the PVS of the various animals were similar despite the different sizes of the animals. The staining properties of their PVS were also same. Thus, the PVS seemed a universal anatomic structure of animals like blood or lymph vessels. In this presentation we propose the methods to detect and observe the PVS network throughout an animal’s entire body and the criteria for identification of the PVS samples. In addition, we introduce a clinical palpation technique which was developed or inspired by the motility characteristics of the PVS.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Fatigue is a common and debilitating late-term effect of breast cancer that is associated with poor sleep and decreased quality of life, yet therapies remain limited. Acupressure has reduced fatigue in previous small studies, but rigorous clinical trials are needed. Here we sought to investigate if 6 weeks of 2 types of self-administered acupressure improved fatigue, sleep, and quality of life vs usual care in breast cancer survivors and to determine if changes were sustained during a 4-week washout period. Our primary outcome was change in the Brief Fatigue Inventory score from baseline to weeks 6 and 10. Secondary analyses were sleep (Pittsburgh Sleep Quality Index) and quality of life (Long-Term Quality of Life Instrument). We enrolled a total of 424 survivors of stages 0 to III breast cancer who had completed cancer treatments at least 12 months previously were screened, and 288 were randomized, with 270 receiving relaxing acupressure (n = 94), stimulating acupressure (n = 90), or usual care (n = 86). At week 6, the percentages of participants who achieved normal fatigue levels (Brief Fatigue Inventory score < 4) were 66.2% (49 of 74) in relaxing acupressure, 60.9% (42 of 70) in stimulating acupressure, and 31.3% (26 of 84) in usual care. At week 10, a total of 56.3% (40 of 71) in relaxing acupressure, 60.9% (42 of 69) in stimulating acupressure, and 30.1% (25 of 83) in usual care continued to have normal fatigue. These effects were statistically significant and clinically relevant. At neither time point were the 2 acupressure groups significantly different. Relaxing acupressure, but not stimulating acupressure, also showed significant improvements in sleep quality compared with usual care at week 6, but not at week 10. Only relaxing acupressure significantly improved quality of life vs usual care at weeks 6 and 10. We conclude that both acupressure arms significantly reduced persistent fatigue compared with usual care, but only relaxing acupressure had significant effects on sleep quality and quality of life. Relaxing acupressure offers a possible low-cost option for managing symptoms in cancer survivors.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Laser acupuncture have a long history since it was firstly performed by Plog in 1973. The principle of laser acupuncture is a combination of biostimulation of photobiomodulation (Low level laser therapy (LLLT)), and acupuncture specific effects. As shown in many reviews, one of the most common indications is pain control and recovery of function in musculo-skeletal disorders: low back pain, shoulder pain, ankle sprain, tendon lesion, osteoarthritis, plantar fasciitis, postoperative recovery, trigeminal neuralgia and etc. In addition, many indications in the pediatric area are being reported: nocturia, asthma, bronchitis, rhinitis, sinusitis, postoperative nausea and vomiting, various pediatric pain disorders, pediatric headaches, and etc. It is also used in neyurogical disease such as stroke sequelae, brain injury, headache, migraine. Studies have been reported in dermatological indication, including acne, allergic diseases, burns and skin ulcer. Advantages of laser acupuncture are that there is no pain in low level laser such as indium laser, and there are advantages such as ease of procedure and hygiene safety as well. In the future, with the development of various devices, the scope of the laser acupuncture and moxibustion is expected to expand further.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Objectives This study is a preliminary study to investigate the possibility of clinical study of electric moxibustion in breast cancer patients with upper limb lymphedema. It has been reported that about 20% of patients suffer from chronic lymphedema after breast cancer surgery. In general, compression therapy, physical therapy, mild exercise, and massage are suggested to improve the symptoms. However, if the symptom persists, it can cause stiffness of the shoulder joints and may interfere with the quality of life. Although there has been a series of studies showing that acupuncture may be an effective treatment, invasive stimulations are generally avoided with patients with lymphedema. Therefore, other possible treatment for the chronic lymphedema is needed. Methods To investigate the feasibility of electric moxibustion in the treatment of upper limb lymphedema, subjects who completed primary cancer treatment at least 6 months ago and has more than 10 mm differences of arm circumference between both upper limbs were included in this study. All subjects were assigned to the treatment group. Subjects were treated with 16 times (30 minutes / times) of electric moxibustion for 8 weeks and was evaluated at baseline, week 5, 9 and 13. For the outcome measures, circumferences of upper limbs, edema index, shoulder range of motions (ROM) and quality of life were assessed before and after the study. Results Eight female participants who completed the study were included in the analyses. The mean age was 50.9 (ranging from 44 to 62) and the mean body mass index was 24.5mg/k2 (ranging from 19.8 to 31.4). Lymphedema patients in dominant side of the upper limb were 3 (37.5%). After 8 weeks of electric moxibustion treatment, the difference in the circumference between both arms decreased significantly. The mean reduction of circumference difference was 8.38mm (95% CI, 0.98-15.77; p = 0.032). The mean difference in flexion ROM between both arms was 13.58 degrees (95% CI, 4.30-22.85; p = 0.011). There were 6 adverse events and most of them were irrelevant to the treatment (upper respiratory infection, back pain, etc.). Only one participant had mild burn on the acupuncture point. Conclusion Here we demonstrate for the first time that electric moxibustion treatment is a feasible treatment for upper-limb lymphedema after breast cancer surgery. We found that electric moxibustion may reduce difference in upper limb circumference and improve shoulder ROM. Future comparative clinical trial is needed to confirm the clinical effectiveness of the treatment.

    Abstract
  • Research Article2020-04-08
    JAMS

    Abstract : Perimenopausal depression is caused by the impaired function of the ovarium before menopause and with a series of symptoms. Electroacupuncture (EA) therapy has been demonstrated to improve clinically depression. However, the mechanism underlying its therapeutic activity remains unknown. This study used chronic unpredictable mild stress (CUMS) combined with bilateral ovariectomy (OVX) to establish a rat model of perimenopausal depression, and investigated the effects of EA treatment on the hippocampal neural proliferation. Four-week EA treatment at three points including “Shenshu” (BL 23), “Baihui” (DU 20), and “Sanyinjiao” (SP 6) simultaneously ameliorated depression-like behaviors in rats with CUMS and OVX, whereas rescued the decreased serum level of estrogen (E2) and prevented the increased serum levels of gonadotropin-releasing hormone and luteinizing hormone. EA treatment ameliorated CUMS and OVX-induced alterations of glycogen synthase kinase-3β and β-catenin mRNA levels, β-catenin and phosphorylated β-catenin (p-β-catenin) protein levels. The results showed that EA treatment promoted hippocampal neural proliferation in perimenopausal depression rats via activating the Wnt/β-catenin signaling pathway, indicating that EA may represent an efficacious therapy for perimenopausal depression.

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  • Research Article2019-06-01

    Transcutaneous Electrical Stimulation of PC5 and PC6 Acupoints Modulates Autonomic Balance in Heart Transplant Patients: A Pilot Study

    Beatriz R. Moreira1, Alice P. Duque2, Carole S. Massolar2, Rodrigo de Lima Pimentel2, Mauro F.F. Mediano34, Tereza C.F. Guimarães3, Luiz F. Rodrigues Jr.12*

    Abstract : The increased resting heart rate (HR) in heart transplant patients is associated with enhanced metabolic demand, the potential for fatigue, and lower quality of life. In the present study, we hypothesized that transcutaneous electrical acupoint stimulation (TEAS) could modulate autonomic balance and reduce resting HR in these patients. A single-arm clinical trial was conducted with patients aged > 18 years, at ambulatorial accompaniment after heart transplantation, who were submitted to a single TEAS (40 minutes at pericardium channel acupoints PC5 and PC6). The arterial blood pressure and RR interval were recorded from 20 minutes before to 20 minutes after TEAS. The RR intervals were used to calculate HR variability (HRV) and the sympathovagal index. Linear mixed models were used for comparing variables before, during, and after TEAS. The significance level was set as P 

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Objective Chemotherapy-induced peripheral neuropathy (CIPN) is a common dose-limiting side effect of anticancer drugs but lacks an effective treatment strategy. Bee venom or Scolopendra subspinipes (SS) has been used in traditional medicine to treat chronic neuronal diseases. Moreover, pharmacopuncture with BV (BVP) or SS (SSP) produces potent analgesia in humans and experimental animals. In this study, we examined the effect of BVP or SSP into the ST36 acupoint on oxaliplatin-induced mechanical allodynia in mice. Methods Mice were given a single administration of oxaliplatin (10 mg/kg) at day 0. At day 14, BVP or SSP was injected into ST36 acupoint and mechanical withdrawal thresholds for hind paws were measured for 4 hours. Results Acupoint treatment with BVP or SSP significantly reduced mechanical allodynia produced by a single oxaliplatin injection, which was completely prevented by acupoint pre-injection of lidocaine. Intrathecal treatment with yohimbine, an α2-adrenoceptor antagonist, prevented the anti-allodynic effect of BVP or SSP. On the other hand, a high dose of clonidine, suppressed oxaliplatin-induced mechanical allodynia, but produced severe side effects including hypotension, bradycardia, and motor impairment. Combination of BVP or SSP with a lower dose of clonidine produced a comparable analgesic effect without side effects. Conclusion Our findings demonstrate that BVP or SSP produces an analgesic effect in oxaliplatin-induced pain via neuronal conduction at the acupoint and activation of spinal α2-adrenoceptors. Moreover, a combination of low-dose clonidine with BVP or SSP represents a novel and safe therapeutic strategy for chemotherapy-induced chronic pain.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Sports injuries are very common, not only among professional athletes, but moreso among those professionals and students who would like to maintain an active lifestyle but only have time during weekends for their favorite sports. These athletic injuries may range from minor sprains to actual muscle tears that require weeks of anti-inflammatory treatment. Age-old acupuncture and moxibustion techniques can actually provide better and faster pain relieving methods that can also facilitate healing with minimal intervention. After the initial application of sports medicine protocols for the acutely injured athlete, immediate daily follow up treatments with moxybustion and acupuncture helps in getting the athlete back to training earlier and more comfortably than any medication currently employed by the conventional physiatrist. A perfect example of the multiplicatory effect of east meets west in the field of sports medicine.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Objectives We investigated the efficacy and safety of thread-embedding acupuncture (TEA) for chronic low back pain (LBP) in a randomized controlled pilot trial with the aim of laying the foundation for a large-scale randomized controlled trial on this topic. Methods Forty participants were recruited for two-arm, assessor-blinded randomized controlled pilot trial. The participants were randomly allocated to a TEA group (experimental group) or an acupuncture group (control group). The TEA group received TEA once every 2 weeks for 8 weeks (four sessions in total), while the acupuncture group received acupuncture twice per week for 8 weeks (16 sessions in total). The primary outcome was the visual analog scale (VAS) score for pain and the secondary outcomes were short-form McGill Pain Questionnaire (SF-MPQ) and Oswestry Disability Index (ODI) scores. Assessments were performed at screening and at 2, 4, 6, 8, and 10 weeks after treatment initiation (the 10-week assessment was conducted at 2 weeks after treatment cessation). Results Of the 40 participants, 36 completed the study and four dropped out. Both the TEA group and the acupuncture group showed significant improvements in VAS, SF-MPQ, and ODI scores in a time-dependent manner. Furthermore, with regard to ODI, a significant interaction between group and time was observed, with the two groups exhibiting a different pattern of change at 8 weeks according to contrast analysis with Bonferroni’s correction. No serious adverse event occurred, and hematological and biochemical test findings were within normal limits. Conclusion This pilot study has provided basic data for a larger clinical trial to demonstrate the efficacy and safety of TEA for chronic LBP.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : ALS , often referred to as “ Lou Gehring’s Disease,” is a progressive disorder that causes degeneration of the motor neurons in the spinal cord, brain stem, and motor cortex that control the voluntary muscle movement. The average ALS patient’s life span is less than three years from when symptoms first appear. Unfortunately ALS is progressive disease until death. The significant symptoms of ALS include muscle spasticity, muscle weakness, paralysis, fasciculation, impaired speaking, swallowing and breathing. Acupuncture treatments showed the high correlation with K-ALSFRS-R score and Sa-am acupuncture lung tonification. And Sa-am acupuncture lung tonification would be more effective on early stage of ALS disease. We found that Pharmacopuncture of Bee venom, Scolopendrid and Hominis Placenta attenuates neuroinflammatory events and extends survival in ALS models. We observed that Mecasin, a novel antineuroinflammatory Agent, inhibits neuroinflammatory responses and Mecasin exerts a neuroprotective effect by ameliorating oxidative stress via heme oxygenase-1 upregulation. According to analysis of clinical trial, the low dose or high dose mecasin group showed better results than the riluteck control group.

    Abstract

Journal Info

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
February, 2021
Vol.14 No.1

pISSN 2005-2901
eISSN 2093-8152

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  • Erratum2020-12-01
  • Research Article2020-08-01

    Abstract : Background: Uremic pruritus is a common boring complaint in patients suffering from chronic renal failure. Owing to cost and the side-effects of medications, complementary therapies are more attractive for pruritus treatment. Objectives: The aim of this study is to determine the effect of acupressure on the severity of pruritus and some laboratory parameters in patients undergoing hemodialysis. Materials and methods: The present clinical trial was conducted on 90 patients undergoing hemodialysis who were allocated in intervention, sham control, and negative control groups (30 in each group). Pressure was applied on SP6, SP10, ST36, and LI11 points in the intervention group and on ineffective points for the sham control group. The negative control group received routine care. The severity of pruritus was measured using the numeric rating scale before, two weeks, and five weeks after intervention. The laboratory parameters were measured before and after the intervention. Results: There was a significant reduction in the severity of pruritus over the course of the study in the intervention and sham control groups (p = 0.001). In addition, significant differences were observed at the end of the intervention in terms of serum phosphorus (p = 0.045) and parathyroid hormone (p = 0.004) levels between groups. Conclusion: Acupressure can improve the severity of pruritus dramatically in hemodialysis patients. It can also reduce serum phosphorus and parathyroid hormone levels, which affect pruritus, significantly. Therefore, this simple and inexpensive intervention may be recommended for reducing uremic pruritus among patients undergoing hemodialysis.

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  • Research Article2020-06-01

    Effectiveness of Acupuncture in Dental Surgery: A Randomized, Crossover, Controlled Trial

    Maria L.B. Gil 1, *, Luide M.R.F. Marinho 2, Márcio de Moraes 2, Ronaldo S. Wada 1, Francisco C. Groppo 3, Jorge E. Sato 4, Maria L.R. de Sousa 1, **

    Abstract : Objectives: The objective of this crossover clinical study was to evaluate the effectiveness of Energy Regulation with Acupuncture in clinical occurrences in impacted lower third molar surgeries. Methods: The sample consisted of 22 patients with two impacted third molars, in symmetrical position; divided into two groups: Test Group (TG) with Real Energy Regulation Group and Sham Group (SG) with Acupuncture without Energy Regulation function. The extraction was performed 30 days apart. Energy flow (Ryodoraku Method) and energy regulation performed before extraction were measured. Heart Rate (HR) and Blood Pressure (BP) were evaluated before and after energy regulation and after surgery, residual edema was measured by facial measurements (angle of the mandible to tragus (A-T); angle of the mandible to labial commissure (A-LC); angle of the mandible to the wing of the nose (A-WN); angle of the mandible to the corner of the eye (A-CE); angle of the mandible to the chin (A-C); and mouth opening by the interincisal distance, before and after seven days of surgery. To quantify intraoperative bleeding (ml), blood was aspirated along with the saline solution using a portable vacuum pump adaptor. The amount of saline solution used was subtracted from the final amount of aspirated fluid. Results: Mean of bleeding was lower in TG (p = 0.0392). There were significant differences between groups in facial distances: A-LC (p = 0.010), A-WN (p = 0.030) and A-C (p = 0.008). Conclusion: Energy regulation with real acupuncture was effective in reducing postoperative residual edema and intraoperative bleeding.

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  • Erratum2020-12-01
  • 2020-04-01
  • Research Paper2019-08-01

    Effects of Acupressure on Fatigue in Patients with Cancer Who Underwent Chemotherapy

    Atefeh Ghanbari Khanghah1, Moloud Sharifi Rizi2*, Bahram Naderi Nabi3, Masoumeh Adib4, Ehsan Kazem Nejad Leili5

    Abstract : Fatigue is the commonest symptom in cancer patients; despite high levels of clinically significant persistent cancer-related fatigue, few treatments are currently available. The aim of this study was to evaluate the efficacy of acupressure on fatigue in patients with cancer who underwent chemotherapy. The study was designed as a randomized and controlled trial. Ninety samples were selected using the convenience sampling method, and random block sampling was used for allocation of groups (30 for each group). The three groups were similar by age and gender. The experimental group underwent acupressure at the Zusanli (ST-36), Hegu (LI-4), and Sanyingjiao (SP-6), whereas sham pressure was used in the placebo group and no intervention was applied in the control group; the level of fatigue of participants in three groups was calculated in three phases, before, during, and after chemotherapy. Data were analyzed using SPSS, version 22. The results showed that the mean of visual analog score of fatigue is significantly different in three groups at the end of chemotherapy (p = 0.021). The mean visual analog score of fatigue in the acupressure group was meaningfully lower than that in the control group after chemotherapy (p = 0.028). Results of this investigation showed that acupressure has short-term effectiveness on the cancer-related fatigue of patients undergoing chemotherapy.

    Abstract
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