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  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Essential hypertension a multifactorial pathophysiological condition involves many systems including the sympathetic nervous system. Increased sympathetic activity contributes to the elevation of blood pressure during sympathoexcitatory cardiovascular reflex responses as well as in hypertension. We have shown that electroacupuncture (EA) reduces sympathoexcitation and both reflex elevations of blood pressure and hypertension in animals and humans. In addition, EA reduces sympathoexcitatory blood pressure responses in hypertensive subjects. Elevated blood pressure also is reduced with manual acupuncture and moxibustion. Hypothalamic to medullary cardiovascular regions in the brain process the actions of EA, manual acupuncture and moxibustion involving specific mechanisms during sympathoexcitation. Recent investigations focused on the peripheral mechanisms at the acupoint site during EA, manual acupuncture and moxibustion. We hypothesized that the transient receptor potential vanilloid (TRPV1) peripheral local mechanism at the acupoint site is involved in the blood pressure lowering effect. In anesthetized rats using neurophysiology, siRNA, pharmacology, immunohistochemistry and hemodynamic responses, our data show that peripheral local TRPV1 participates during the blood pressure lowering effect with manual acupuncture and heat sensitive moxibustion but not EA. Mechano sensitive TRPV1 contributes to manual acupuncture activating peripheral somatosensory nerves. In contrast, EA-induced increase of somatosensory activity is not affected by blockade of TRPV1 at the site of acupoint. Similarly, TRPV1 knockdown in dorsal root ganglia (DRG) reduced activation of the peripheral DRG neurons during manual acupuncture but not EA. Sympathoexcitatory increase in blood pressure is reduced by acupoint specific moxibustion through local heat sensitive TRPV1 but not EA. In aggregate, EA, manual acupuncture and moxibustion applied at specific acupoints employ different peripheral local mechanisms to reduce elevated blood pressure and over-excitation of sympathetic activity.

    Abstract
  • Abstract : Headache disorders are burdensome, both in terms of the number of people they affect, and in terms of associated healthcare spending. This report presents a 36-year-old female admitted to a tertiary university hospital with a primary complaint of intractable headache, caused by a combination of medication overuse headache, and headache secondary to aseptic meningitis. During her hospital stay, opioid analgesic doses were initially increased without success in an attempt to control her headache. Despite multiple medication trials the patient's headache failed to improve. On day ten of her hospitalization, she underwent a thirty-minute acupuncture session which resulted in immediate relief of her headache. She received one more acupuncture treatment the following day and was discharged to an acute inpatient rehabilitation facility on a vastly reduced dose of opioids. Instructions on how to taper the remaining opioids were provided, and the patient was scheduled for outpatient acupuncture therapy sessions for further headache management. This report demonstrates the importance of recognizing acupuncture as a viable treatment option for medication overuse headache and for headache secondary to systemic diseases such as aseptic meningitis. Furthermore, acupuncture should also be considered as a nonpharmacological modality to be used when tapering a patient off of high doses of opioids.

    Abstract
  • Research Article2020-06-01

    Electroacupuncture Promotes Neural Proliferation in Hippocampus of Perimenopausal Depression Rats via Wnt/β-Catenin Signaling Pathway

    Qin Jing , Lu Ren*, †, Xue Deng, Nan Zhang, Martin Fu, Ge Wang, Xi-Rong Jiang, Shu-Ru Lin, Cai-Rong Ming

    Abstract : Background and Objective: Perimenopausal depression is caused by the impaired function of the ovarium before menopause and with a series of symptoms. Electroacupuncture (EA) therapy has been demonstrated to improve clinically depression. However, the mechanism underlying its therapeutic activity remains unknown. This study aimed to investigat the effects of EA treatment on the hippocampal neural proliferation through Wnt signaling pathway. Methods: Chronic unpredictable mild stress (CUMS) combined with bilateral ovariectomy (OVX) were used to establish a rat model of perimenopausal depression. The open field test (OFT) and sucrose preference test (SPT) were used to assess depression-like behaviors in rats. ELISAs were used to measure estrogen (E2), luteinizing hormone (LH) and gonadotropin-releasing hormone (GnRH) levels in the serum. RT-PCR and Western blot assay were utilized for measuring the mRNA expressions and protein expressions of GSK-3β/β-catenin. Results: Four-week EA treatment at three points including “Shenshu” (BL23), “Baihui” (GV20) and “Sanyinjiao” (SP6) simultaneously ameliorated depression-like behaviors in rats with CUMS and OVX, whereas rescued the decreased serum level of E2 and prevented the increased serum levels of GnRH and LH. EA treatment ameliorated CUMS and OVX-induced alterations of glycogen synthase kinase-3b (GSK3b) and β-catenin mRNA levels, β-catenin and phosphorylated β-catenin (p-β-catenin) protein levels. Conclusions: The results showed that EA treatment promoted hippocampal neural proliferation in perimenopausal depression rats via activating the Wnt/β-catenin signaling pathway, indicating that EA may represent an efficacious therapy for perimenopausal depression.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Objectives Knee pain is the illness that has ranked top in the medical cost and visit number in the Korean Medicine (KM) institutions. With the trend that the older aged population is increasing, it is predicted that people with knee pain is increasing resulting in the life quality degradation and the high social costs. In this situation, KM has strength in the clinical practice of knee pain. The purpose of Clinical Practice Guideline (CPG) was to enhance the reliability of KM by reinforcing the evidence with objective and rational verification, to develop a standardized clinical guideline, and to make a reasonable judgment in the treatment of knee pain. Methods We assembled committee with experts and established development plan. Committee set up key questions and searched relevant evidence comprehensively by using following databases: Pubmed, Ovid-EMBASE, Cochrane library, CNKI, OASIS, and NDSL. The evidence were reviewed according to the pre-defined selection/exclusion criteria, and the guideline draft was made by using the quality of evidence (4 levels: High, Moderate, Low, and Insufficient) and the grade of recommendation (5 grades: A, B, C, D, and GPP) based on the GRADE method. Official consensus was obtained through the following steps: 1) we used the Delphi method to reach agreement on the draft. 2) draft was further revised through external review and produced into the final guideline. 3) guideline obtained confirmation from relevant academic societies. Results We classified knee pain into 4 categories (knee osteoarthritis, knee rheumatoid arthritis, soft tissue injury, and other type) and made guideline according to several KM treatments (e.g. manual acupuncture, electroacupuncture, warm or fire needle acupuncture, moxibustion, herbal medicine, pharmacopuncture, acuputomy, cupping, and others). Among them, it was confirmed that pharmacopuncture had moderate evidence and B recommendation level (high considerable) on knee osteoarthritis. To complement the existing CPG published in 2017, we will expand KM treatments (e.g. physiotherapy, chuna, and manipulation therapy), focus on the disease name (e.g. knee osteoarthritis not knee pain), and create evidence through direct execution of randomized clinical trial. Conclusion CPG is expected to provide objectivity, rationality, and standard of KM treatment for knee pain by continuous research and supplementation.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : Visceral pain produces hypersensitive spots on the skin (neurogenic spots), caused by cutaneous neurogenic inflammation, in the dermatome that overlaps with visceral afferent innervation; the spots can be visualized experimentally on the skin by extravasation of Evans blue dye. Here, we show that an acupoint is one form of neurogenic inflammation on the skin. Various studies have demonstrated that acupoints show mechanical hypersensitivity and have high electrical conductance. Stimulation of acupoints produces needling sensations caused by activation of small afferent fibers and therapeutic effects on the associated visceral organs, likely due to the release of endogenous opioids. The present study provides experimental evidence that neurogenic spots exhibit all the characteristics of the acupoints listed above. In addition, the stimulation of neurogenic spots by electrical, mechanical, or chemical means alleviated pathological conditions in rat colitis and hypertension models via endogenous opioid systems. Blocking NK1R in neurogenic spots prevented the acupuncture effects. Elevated substance P and CGRP caused low electrical resistance in neurogenic spots as well as acupoints. Our results demonstrate that an acupoint is identical to a neurogenic inflammatory spot on the skin, which is produced by activation of somatic C-fiber terminals in abnormal conditions of visceral organs. This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT)(No.2018R1A5A2025272 and 2018R1D1A1B07046196), KBRI basic research program through Korea Brain Research Institute funded by Ministry of Science and ICT (18-BR-03) and Korea Institute of Oriental Medicine (KIOM) K18181.

    Abstract
  • Research Paper2019-12-01

    Comparative Analgesia Between Acupuncture and Dipyrone in Odontalgia

    Talita B. de Almeida, Vera L.R. Zotelli, Ronaldo S. Wada, Maria L.R. Sousa*

    Abstract : The aim of this study was to assess whether the effectiveness of acupuncture is similar to the use of analgesics in the management of toothache. The research included 56 volunteers who were divided into 4 groups: Real Acupuncture group, Placebo Acupuncture group, Real Dipyrone group, and Placebo Dipyrone group. The interventions of the study were performed before the dental care. Inclusion criteria were toothache of pulpal origin with pain scale (Visual Analogue Scale) above 4, absence of medication for the pain, and aged over 18 years. The Real Acupuncture volunteers received a session of acupuncture using piercing needles, while volunteers from the Placebo Acupuncture group received an acupuncture session using non-piercing sham needles. Volunteers from the Real Dipyrone group received a dipyrone tablet and the Placebo Acupuncture group received a tablet with no active ingredient. Before any therapeutic intervention, we collected samples from the volunteers’ saliva to analyze the salivary cortisol, the volunteers rated the intensity of their pain using VAS, and we measured their energy level by the Ryodoraku method. After 20 minutes of treatment, all the volunteers’ analysis parameters were collected again. The Real Acupuncture group presented a greater reduction of VAS than the reduction obtained by the Real Dipyrone group (p

    Abstract
  • Research Article2020-06-01
    JAMS

    Abstract : ObjectivesThe aim of this study is to determine the effect of acupressure on the severity of pruritus and some laboratory parameters in patients undergoing hemodialysis.Materials and MethodsThe present clinical trial was conducted on 90 hemodialysis patients. Pressure was applied on SP6, SP10, ST36, and LI11 points in the intervention group and on ineffective points for the sham control group. The severity of itching was measured using the Numeric Rating Scale.ResultsThere was a significant reduction in the severity of pruritus over the course of the study in the intervention and sham control groups (P=0.001). Also significant differences were observed at the end of the intervention in terms of serum phosphorus (P=0.045) and parathyroid hormone (P=0.004) levels between groups.ConclusionAcupressure can improve the severity of pruritus dramatically in hemodialysis patients, but has no effect on laboratory parameters, except for serum phosphorus and parathyroid hormone levels.

    Abstract
  • International Scientific Acupuncture and Meridian Symposium (ISAMS) 2019 Conference Abstracts2020-04-01
    JAMS

    Abstract : While several studies have found that chronic pain is characterized by increased cross-network connectivity between salience, sensorimotor, and default mode (DMN) networks, a large sample-size investigation allowing a more reliable evaluation of somatotopic specificity and subgroup analyses with linkage to clinical pain intensity has been lacking. We enrolled healthy adults and a large cohort of patients (N=181) suffering from chronic low back pain (cLBP). To specifically link brain connectivity with clinical pain intensity, patients were scanned at baseline and after performing physical maneuvers that exacerbated pain. Compared to healthy adults, cLBP patients demonstrated increased connectivity between the functionally-localized back representation in primary somatosensory cortex (S1back) and both salience and DMN networks. Pain exacerbation maneuvers increased S1back connectivity to salience network regions, but decreased connectivity to DMN, with greater pain intensity increase associated with greater shifts in these connectivity patterns. Furthermore, only in cLBP patients reporting high pain catastrophizing, DMN connectivity was increased to a cardinal node of the salience network, anterior insula cortex, which was correlated with increased post-maneuver pain in this cLBP subgroup. Hence, increased information transfer between salience processing regions, particularly anterior insula, and DMN may be strongly influenced by pain catastrophizing. Increased information transfer between salience network and S1 likely plays an important role in shifting nociceptive afference away from self-referential processing, re-allocating attentional focus and affective coding of nociceptive afference from specific body areas. These results demonstrate S1 somatotopic specificity for cross-network connectivity in encoding clinical back pain, and moderating influence of catastrophizing for DMN/insula connectivity.

    Abstract
  • Research Paper2019-10-01

    Stimulation of Bladder Acupoints by Cloprostenol for Treating Back Soreness in Athletic Horses

    Eldessouky Sheta1*, Haithem Farghali1, Safwat Ragab2, Nagwa Hassan3, Asmaa El-Sherif4

    Abstract : Twenty-five Thoroughbred jumper geldings suffered back soreness with poor performance, and 5 control horses were assessed by archived computer data, clinical examination, and laboratory analyses of complete blood picture, serum enzymes, and cortisol level, before and after cloprostenol-pharmacopuncture. The 25 diseased horses before therapy showed significant increases in aspartate aminotransferase and creatine phosphokinase with clinical pains scored mild in 15 horses, moderate in 9 horses, and severe in one horse, without changes in the hormonal and hematological data. After therapy, they responded by an increase of heart rate (57.8 ± 4.3 bpm), body temperature (38.5 ± 0.7°C), respiration rate (28.3 ± 2.1 bpm), and capillary refilling time (CRT) (1.0 ± 0.0). On the 2nd day, a significant decrease in the mean levels of aspartate aminotransferase and creatine phosphokinase (P = 0.001) was detected, while on the 4th day, they mimed the level of the 5 controls, and on the 6th day, they showed a significant decrease (P = 0.002). The serum cortisol level showed a significant increase on the 6th day of treatment (P = 0.013). The blood picture showed significant increases in red blood cells, mean corpuscular volume, platelets, white blood cells, hemoglobin, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, mean platelet volume, platelet distribution width, lymphocytes, plateletcrit, and large platelet concentration ratio (P 

    Abstract
  • Abstract : Background: The primo vascular system (PVS) has been difficult to detect due to its small diameter and translucent features of the threadlike network. Thus, contrast-enhancing dyes including Alcian blue, Trypan blue and Janus green B had to be used for finding and taking out PVS from rat and mouse. Objective: Generation of monoclonal antibodies (mAbs) against PVS of rat was intended to use as a detector for PVS and a biological tool for functional study of PVS. Materials and methods: Primo vessel (PV) and Primo node (PN) were isolated from organ surfaces of rat and then their proteins were isolated and injected into mouse as an immunogen. The classical traditional method was applied for production of mAbs against PVS. The various techniques, such as cell fusion, screening of hybridoma, ELISA, Western blotting (WB), immunofluorescence microscopy (IF), and limiting dilution, were used to generate mAbs against PVS. Results: Among 16 mAbs generated, 4 representative mAbs were characterized with their specificities in ELISA, WB, and IF. α-rPVS-m1-1 and α-rPVS-m4-6 had strong binding affinities to PVS in both ELISA and WB but did not show specificities in IF at all. On the contrary, α-rPVS-m3-2 and α-rPVS-m3-4 almost did not respond in WB but had strong binding affinities in ELISA and specificities in IF. Two mAbs stained predominantly at extra cellular matrix and cell membrane of PVS of rat in IF, and they were able to discriminate PVS from blood vessel (BV) and lymphatic vessel (LV). Conclusions: 4 representative mAbs against PVS of rat were characterized by ELISA, WB, and IF. α-rPVSm3-2 and α-rPVS-m3-4, which had strong specificities in IF, can be used as a tool in discriminating PVS from other similar tissues and in elucidate biological function of PVS.

    Abstract

Journal Info

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
February, 2021
Vol.14 No.1

pISSN 2005-2901
eISSN 2093-8152

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  • Research Article2020-10-01

    Impact of Acupuncture Intervention on the Pain Intensity of Patients Treated at a Tertiary Hospital in Brazil

    Natália F. Valente 1, *, Eliezer de Sousa Cardoso 2, Juliana A. da Silva Rezende 2, Jeferson A. Santos 3

    Abstract : Background: Nowadays, in western societies, acupuncture is widely used over the control of pain and this analgesic approach is still the most studied aspect of acupuncture. Several studies have shown that most patients go through a significant pain decrease soon after the first sessions of acupuncture. Objectives: This research has as a goal the evaluation on the effect of acupuncture treatment regarding the relief of pain intensity of different etiologies, through the visual analog scale. Methods: This research constitutes a retrospective, descriptive study, carried out with 449 patients attended in the Institute Hospital de Base of the Federal District, in Brasilia citye Brazil. Every data was gathered from our own form, with detailed clinical history which included age, marital status, work activity, reason for referral to acupuncture, main and secondary complaints, pain intensity evaluated by the Visual Analog Scale (VAS), number of sessions completed and drug therapy. All data was organized in the Microsoft Excel and processed in the Statistical Package for the Social Sciences (SPSS), version 20.0. Results: Regarding the main complaint, the mean pain decreased from 7.3 (initial VAS) to 3.2 (final VAS), a reduction that meant more than 50% relief in pain intensity. This 50% reduction in initial pain was also observed in secondary complaints (initial VAS = 6.5 and final VAS = 3.1). Conclusions: The results of this research suggest that acupuncture treatment was effective in relieving pain intensity, providing a 50% reduction on the visual analogue scale, in relation to painful complaints of different etiologies.

    Abstract
  • Research Article2020-04-01

    Abstract : The prevalence of elderly dementia and work-related back neck pain is high, and so is the associated cost. Is dementia, which can take 20–30 years to develop, linked to musculoskeletal problems earlier in life? Classic Chinese Medicine Governor Vessel (GV) theory has in fact detailed a correlation, but its acceptance appears to be influenced and even limited by some inconsistences in the literature. This study involved a nationwide online questionnaire of 381 practicing acupuncturists to investigate GV's usage and impact on brain function. The survey covers areas of the acupuncturist's experience, perception of GV usage, related symptoms, needling concerns and correlations with brain function. 39 completed questionnaires were returned, of which 38 were GV users, and nearly three quarters of respondents accepted that GV influences brain function. Only two respondents rejected this influence, but here several inconsistencies were found. The associations with GV were predominately found to be with the spine, kidney, and brain. Indications from practitioners suggested numerous forms of clinical usage of GV, predominately on spinal, cognitive and qi-blood movement issues. GV influence on the brain was accepted by most respondents with only a few expressing uncertainty. Further worldwide, larger population, in-depth studies and trials are needed to fully establish and expand upon these findings.

    Abstract
  • Research Paper2019-08-01

    Effects of Acupressure on Fatigue in Patients with Cancer Who Underwent Chemotherapy

    Atefeh Ghanbari Khanghah1, Moloud Sharifi Rizi2*, Bahram Naderi Nabi3, Masoumeh Adib4, Ehsan Kazem Nejad Leili5

    Abstract : Fatigue is the commonest symptom in cancer patients; despite high levels of clinically significant persistent cancer-related fatigue, few treatments are currently available. The aim of this study was to evaluate the efficacy of acupressure on fatigue in patients with cancer who underwent chemotherapy. The study was designed as a randomized and controlled trial. Ninety samples were selected using the convenience sampling method, and random block sampling was used for allocation of groups (30 for each group). The three groups were similar by age and gender. The experimental group underwent acupressure at the Zusanli (ST-36), Hegu (LI-4), and Sanyingjiao (SP-6), whereas sham pressure was used in the placebo group and no intervention was applied in the control group; the level of fatigue of participants in three groups was calculated in three phases, before, during, and after chemotherapy. Data were analyzed using SPSS, version 22. The results showed that the mean of visual analog score of fatigue is significantly different in three groups at the end of chemotherapy (p = 0.021). The mean visual analog score of fatigue in the acupressure group was meaningfully lower than that in the control group after chemotherapy (p = 0.028). Results of this investigation showed that acupressure has short-term effectiveness on the cancer-related fatigue of patients undergoing chemotherapy.

    Abstract
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  • Erratum2020-12-01
  • Research Article2020-10-01

    Impact of Acupuncture Intervention on the Pain Intensity of Patients Treated at a Tertiary Hospital in Brazil

    Natália F. Valente 1, *, Eliezer de Sousa Cardoso 2, Juliana A. da Silva Rezende 2, Jeferson A. Santos 3

    Abstract : Background: Nowadays, in western societies, acupuncture is widely used over the control of pain and this analgesic approach is still the most studied aspect of acupuncture. Several studies have shown that most patients go through a significant pain decrease soon after the first sessions of acupuncture. Objectives: This research has as a goal the evaluation on the effect of acupuncture treatment regarding the relief of pain intensity of different etiologies, through the visual analog scale. Methods: This research constitutes a retrospective, descriptive study, carried out with 449 patients attended in the Institute Hospital de Base of the Federal District, in Brasilia citye Brazil. Every data was gathered from our own form, with detailed clinical history which included age, marital status, work activity, reason for referral to acupuncture, main and secondary complaints, pain intensity evaluated by the Visual Analog Scale (VAS), number of sessions completed and drug therapy. All data was organized in the Microsoft Excel and processed in the Statistical Package for the Social Sciences (SPSS), version 20.0. Results: Regarding the main complaint, the mean pain decreased from 7.3 (initial VAS) to 3.2 (final VAS), a reduction that meant more than 50% relief in pain intensity. This 50% reduction in initial pain was also observed in secondary complaints (initial VAS = 6.5 and final VAS = 3.1). Conclusions: The results of this research suggest that acupuncture treatment was effective in relieving pain intensity, providing a 50% reduction on the visual analogue scale, in relation to painful complaints of different etiologies.

    Abstract
  • Case Report2019-06-01

    Concurrent Effects of Dry Needling and Electrical Stimulation in the Management of Upper Extremity Hemiparesis

    Maryam S. Ghaffari1, Ardalan Shariat1*, Roshanak Honarpishe2, Azadeh Hakakzadeh1, Joshua A. Cleland3, Sepehr Haghighi4, Tohid S. Barghi1

    Abstract : Stroke is one of the leading causes of disability in western countries. A variety of rehabilitation programs for the treatment of patients after stroke have been proposed. We describe the outcomes of a 49-year-old female patient with a 5-year history of right upper extremity hemiparesis after stroke. Physical examination revealed a right wrist extensor strength grade of 1 according to the Medical Research Council Manual Muscle Testing scale, Stage 4 according to the Brunnstrom hand functional recovery, and Grade 1 in finger flexor and in wrist flexor according to the Modified Modified Ashworth Scale system of muscle spasticity. Magnetic resonance imaging taken immediately after the stroke was indicative of an abnormal signal in the left paraventricular and lentiform nucleus. After receiving a single session of dry needling and electrical stimulation, the patient had significant improvement including a strength grade of 3 for the right wrist extensor muscles, Stage 6 according to the Brunnstrom hand functional recovery, and Grade 0 in finger flexor and in wrist flexor according to the Modified Modified Ashworth Scale system of muscle spasticity. This case report found that dry needling combined with electrical stimulation may be effective in hand function recovery, wrist extensor muscles strength, and decreased wrist and finger spasticity.

    Abstract
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